Birthdating analysis of the output of cortical progenitor cells at E14.5 in the wild-type and Dicer null cortex found that there was no significant difference in the number of Brd U positive neurons found in the P0 cortex (Figure 5).
The cerebral cortex as the main centre for all higher cognitive functions develops a layered structure which is essential for its function.
This lamination develops in an inside-out fashion and requires the secreted glycoprotein reelin (., 1999) result in inversion of cortical layers and in abnormally dispersed cells.
In contrast to the well understood role of reelin signalling, relatively little is known about the identity of the reelin).
In addition, the caudomedial wall of the telencephalon, including the cortical hem, is a major source of a CR cell population which is characterized by the expression of reelin, p73, Calretinin and glutamate ( Meyer ., 2006).
Bromodeoxyuridine birthdating was carried out on E11.5 to label S-phase neurons.
The days of injection were chosen because they are at the onset of neurogenesis and axon extension for corticothalamic, thalamocortical, and corticospinal neurons.
Embryonic (E) day 0.5 was assumed to start at midday of the day of vaginal plug discovery. For each marker and each stage, 3–5 different, non-exencephalic embryos were analysed at rostral, medial and caudal levels of the developing cortex.
To distinguish between these possibilities, Brd U birthdating was carried out at E14.5, at the stage of cortical neurogenesis in the wild-type cortex at which the majority of cortical progenitor cells have switched from producing early born, deep layer neurons to generating upper layer neurons .
Regardless of severity, sensorimotor defects are commonly reported.
Sensorimotor information travels through three tracts of the internal capsule: thalamocortical axons, corticothalamic axons, and corticospinal axons.
Moreover, the radial glial network is disturbed in the regions of these clusters.